Part:BBa_K2683027

Elongation Factor- Thermo Stable (EF-Ts) in pSB1C3

Elongation Factor- Thermo stable (EF-Ts) is a prokaryotic elongation factor that acts at the guanine nucleotide exchange factor for EF-Tu, catalyzing the release of GDP from EF-Tu. This enables EF-Tu to bind to a new GTP and catalyze another aminoacyl tRNA addition to the peptide chain [1]. This par is codon optimized for use in Escherichia coli , has a C-terminal His tag for purification purpose and has a T7 promoter, medium strength RBS and double terminator. This part was originally designed by the Lethbridge iGEM in 2017 (http://2017.igem.org/Team:Lethbridge). This part was initially characterized in the pJET plasmid (https://parts.igem.org/Part:BBa_K2443028).

This part was improved by placing the part into the pSB1C3 plasmid.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 88
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]